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t7e1 enzyme  (New England Biolabs)


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    New England Biolabs t7e1 enzyme
    Sidt2 ablation induced the loss of β-cell identity markers in vitro. ( A ) <t>T7E1</t> enzyme validation of Sidt2 −/− INS-1 cells constructed using CRISPR-Cas9 technology (1: Sidt2 +/+ INS-1 cells; 2–3: Sidt2 −/− INS-1 cells). ( B ) Sequencing map of Sidt2 −/− INS-1 monoclonal cells. ( C ) Verification of Sidt2 protein levels in Sidt2 +/+ and Sidt2 −/− INS-1 cells. ( D ) mRNA levels of β-cell markers in Sidt2 +/+ and Sidt2 −/− INS-1 cells. ( E ) Protein expression of β-cell markers in Sidt2 +/+ and Sidt2 −/− INS-1 cells. ( F ) Statistical analysis of data from panel ( E ). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
    T7e1 Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 260 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/t7e1+mutation+assay+engen+mutation+kit/EnGen+Mutation+Detection+Kit/pmc12406273-219-1-4
    Average 96 stars, based on 260 article reviews
    t7e1 enzyme - by Bioz Stars, 2026-10
    96/100 stars

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    1) Product Images from "Sidt2 inhibits islet β-cell dedifferentiation by regulating insulin secretion"

    Article Title: Sidt2 inhibits islet β-cell dedifferentiation by regulating insulin secretion

    Journal: The Journal of Biological Chemistry

    doi: 10.1016/j.jbc.2025.110544

    Sidt2 ablation induced the loss of β-cell identity markers in vitro. ( A ) T7E1 enzyme validation of Sidt2 −/− INS-1 cells constructed using CRISPR-Cas9 technology (1: Sidt2 +/+ INS-1 cells; 2–3: Sidt2 −/− INS-1 cells). ( B ) Sequencing map of Sidt2 −/− INS-1 monoclonal cells. ( C ) Verification of Sidt2 protein levels in Sidt2 +/+ and Sidt2 −/− INS-1 cells. ( D ) mRNA levels of β-cell markers in Sidt2 +/+ and Sidt2 −/− INS-1 cells. ( E ) Protein expression of β-cell markers in Sidt2 +/+ and Sidt2 −/− INS-1 cells. ( F ) Statistical analysis of data from panel ( E ). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
    Figure Legend Snippet: Sidt2 ablation induced the loss of β-cell identity markers in vitro. ( A ) T7E1 enzyme validation of Sidt2 −/− INS-1 cells constructed using CRISPR-Cas9 technology (1: Sidt2 +/+ INS-1 cells; 2–3: Sidt2 −/− INS-1 cells). ( B ) Sequencing map of Sidt2 −/− INS-1 monoclonal cells. ( C ) Verification of Sidt2 protein levels in Sidt2 +/+ and Sidt2 −/− INS-1 cells. ( D ) mRNA levels of β-cell markers in Sidt2 +/+ and Sidt2 −/− INS-1 cells. ( E ) Protein expression of β-cell markers in Sidt2 +/+ and Sidt2 −/− INS-1 cells. ( F ) Statistical analysis of data from panel ( E ). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

    Techniques Used: In Vitro, Biomarker Discovery, Construct, CRISPR, Sequencing, Expressing

    Related Articles

    Polymerase Chain Reaction:

    Article Title: A role for the cystic fibrosis transmembrane conductance regulator in the nitric oxide-dependent release of Cl - from acidic organelles in amacrine cells.
    Article Snippet: .. The PCR product (~394 bp) was then analyzed for indels in the CFTR gene using the T7 endonuclease I (T7E1) mutation assay (EnGen Mutation kit; New England Biolabs). ..

    Article Title: A role for the cystic fibrosis transmembrane conductance regulator in the nitric oxide-dependent release of Cl − from acidic organelles in amacrine cells
    Article Snippet: .. The PCR product (~394 bp) was then analyzed for indels in the CFTR gene using the T7 endonuclease I (T7E1) mutation assay (EnGen Mutation kit; New England Biolabs). ..

    Mutagenesis:

    Article Title: A role for the cystic fibrosis transmembrane conductance regulator in the nitric oxide-dependent release of Cl - from acidic organelles in amacrine cells.
    Article Snippet: .. The PCR product (~394 bp) was then analyzed for indels in the CFTR gene using the T7 endonuclease I (T7E1) mutation assay (EnGen Mutation kit; New England Biolabs). ..

    Article Title: A role for the cystic fibrosis transmembrane conductance regulator in the nitric oxide-dependent release of Cl − from acidic organelles in amacrine cells
    Article Snippet: .. The PCR product (~394 bp) was then analyzed for indels in the CFTR gene using the T7 endonuclease I (T7E1) mutation assay (EnGen Mutation kit; New England Biolabs). ..



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    t7e1 enzyme  (New England Biolabs)
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    New England Biolabs t7e1 enzyme
    Sidt2 ablation induced the loss of β-cell identity markers in vitro. ( A ) <t>T7E1</t> enzyme validation of Sidt2 −/− INS-1 cells constructed using CRISPR-Cas9 technology (1: Sidt2 +/+ INS-1 cells; 2–3: Sidt2 −/− INS-1 cells). ( B ) Sequencing map of Sidt2 −/− INS-1 monoclonal cells. ( C ) Verification of Sidt2 protein levels in Sidt2 +/+ and Sidt2 −/− INS-1 cells. ( D ) mRNA levels of β-cell markers in Sidt2 +/+ and Sidt2 −/− INS-1 cells. ( E ) Protein expression of β-cell markers in Sidt2 +/+ and Sidt2 −/− INS-1 cells. ( F ) Statistical analysis of data from panel ( E ). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
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    Sidt2 ablation induced the loss of β-cell identity markers in vitro. ( A ) <t>T7E1</t> enzyme validation of Sidt2 −/− INS-1 cells constructed using CRISPR-Cas9 technology (1: Sidt2 +/+ INS-1 cells; 2–3: Sidt2 −/− INS-1 cells). ( B ) Sequencing map of Sidt2 −/− INS-1 monoclonal cells. ( C ) Verification of Sidt2 protein levels in Sidt2 +/+ and Sidt2 −/− INS-1 cells. ( D ) mRNA levels of β-cell markers in Sidt2 +/+ and Sidt2 −/− INS-1 cells. ( E ) Protein expression of β-cell markers in Sidt2 +/+ and Sidt2 −/− INS-1 cells. ( F ) Statistical analysis of data from panel ( E ). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
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    t7e1 assay  (New England Biolabs)
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    Sidt2 ablation induced the loss of β-cell identity markers in vitro. ( A ) <t>T7E1</t> enzyme validation of Sidt2 −/− INS-1 cells constructed using CRISPR-Cas9 technology (1: Sidt2 +/+ INS-1 cells; 2–3: Sidt2 −/− INS-1 cells). ( B ) Sequencing map of Sidt2 −/− INS-1 monoclonal cells. ( C ) Verification of Sidt2 protein levels in Sidt2 +/+ and Sidt2 −/− INS-1 cells. ( D ) mRNA levels of β-cell markers in Sidt2 +/+ and Sidt2 −/− INS-1 cells. ( E ) Protein expression of β-cell markers in Sidt2 +/+ and Sidt2 −/− INS-1 cells. ( F ) Statistical analysis of data from panel ( E ). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
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    https://www.bioz.com/product/t7e1+mutation+assay+engen+mutation+kit/EnGen+Mutation+Detection+Kit/pm38315865-216-7-9
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    Image Search Results


    Sidt2 ablation induced the loss of β-cell identity markers in vitro. ( A ) T7E1 enzyme validation of Sidt2 −/− INS-1 cells constructed using CRISPR-Cas9 technology (1: Sidt2 +/+ INS-1 cells; 2–3: Sidt2 −/− INS-1 cells). ( B ) Sequencing map of Sidt2 −/− INS-1 monoclonal cells. ( C ) Verification of Sidt2 protein levels in Sidt2 +/+ and Sidt2 −/− INS-1 cells. ( D ) mRNA levels of β-cell markers in Sidt2 +/+ and Sidt2 −/− INS-1 cells. ( E ) Protein expression of β-cell markers in Sidt2 +/+ and Sidt2 −/− INS-1 cells. ( F ) Statistical analysis of data from panel ( E ). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

    Journal: The Journal of Biological Chemistry

    Article Title: Sidt2 inhibits islet β-cell dedifferentiation by regulating insulin secretion

    doi: 10.1016/j.jbc.2025.110544

    Figure Lengend Snippet: Sidt2 ablation induced the loss of β-cell identity markers in vitro. ( A ) T7E1 enzyme validation of Sidt2 −/− INS-1 cells constructed using CRISPR-Cas9 technology (1: Sidt2 +/+ INS-1 cells; 2–3: Sidt2 −/− INS-1 cells). ( B ) Sequencing map of Sidt2 −/− INS-1 monoclonal cells. ( C ) Verification of Sidt2 protein levels in Sidt2 +/+ and Sidt2 −/− INS-1 cells. ( D ) mRNA levels of β-cell markers in Sidt2 +/+ and Sidt2 −/− INS-1 cells. ( E ) Protein expression of β-cell markers in Sidt2 +/+ and Sidt2 −/− INS-1 cells. ( F ) Statistical analysis of data from panel ( E ). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

    Article Snippet: The T7E1 enzyme (E3321, New England Biolabs) was used to confirm the presence of mutated bases.

    Techniques: In Vitro, Biomarker Discovery, Construct, CRISPR, Sequencing, Expressing